Biochemistry is the science which deals with the chemical composition, the synthesis, the degradation and interaction of compounds found in living organisms. More simply it encompasses understanding of life processes in terms of chemical phenomena. The theme of biochemistry is to correlate the molecular structures with the biological functions. Today, the growth of all other biological sciences such as biotechnology, biology, physiology, genetics, pathology, microbiology, medicine, agriculture etc. is dependent upon biochemistry. Biochemistry has an important role to play in all the life processes and technological developments in the area of food energy public health and pollution control in order to meet the requirement with the rapidly increasing world population in the years to come. Biochemistry is an experimental science.This "Biochemistry Manual" has been written to help researchers, teachers and students for proper understanding and use of the major techniques and methods employed in biochemistry.