Cassie Chambers grew up in eastern Kentucky. She graduated from Yale College, the Yale School of Public Health, the London School of Economics, and Harvard Law School, where she was president of the Harvard Legal Aid Bureau, a student-run law firm that represents low-income clients. Chambers then received a Skadden Fellowship to return to Kentucky to do legal work with domestic violence survivors in rural communities. In 2018, she helped pass Jeanette's Law, which eliminated the requirement that domestic violence survivors pay an incarcerated spouse's legal fees in order to get a divorce. She lives in Louisville with her husband and their son.